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coli BL21(DE3) cells carrying the plasmid pET-BirA (protein sequence: His 6 -SSGLVPRGSH-BirA) 82 (a kind gift from Professor Ying Gao at the authors institute) were induced with 0.1 mM IPTG for 15 h at 18 C

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AFG3L2 inhibits the cancer-promoting function of SLC25A39 by affecting its stability To explore the potential mechanism of SLC25A39 in LUAD progression, we transfected 293 T cells with a Flag-tagged SLC25A39 plasmid (Flag-SLC25A39) and performed co-immunoprecipitation (Co-IP) using Flag antibodies